FGF2 antibody (AA 143-288)

Catalog No. ABIN3042812

The Rabbit Polyclonal anti-FGF2 antibody has been validated for WB, ELISA, ICC, FACS and IF. It is suitable to detect FGF2 in samples from Human. There are 6+ publications available.

Quick Overview for FGF2 antibody (AA 143-288) (ABIN3042812)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-FGF2 Antibody

Binding Specificity: AA 143-288

Purpose: Anti-Fibroblast growth factor 2 FGF2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Fibroblast growth factor 2 FGF2 Antibody (ABIN3042812). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FGF2 recombinant protein (Position: P143-S288).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Doniach, T. : Basic FGF as an inducer of anteroposterior neural pattern. Cell 83: 1067-1070, 1995. 2. Eckenstein, F. P. : Fibroblast growth factors in the nervous system. J. Neurobiol. 25: 1467-1480, 1994.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-FGF2 antibody (ABIN3042812)

Xiong, Sun, Liu, Xu, He et al.: "Effect of CPU-XT-008, a combretastatin A-4 analogue, on the proliferation, apoptosis and expression of vascular endothelial growth factor and basic fibroblast growth factor in human umbilical vein ..." in: Oncology letters, Vol. 11, Issue 1, pp. 491-499, (2016) (PubMed).

Wang, Wang, Yao, Wu, Tang, Gu, Li: "The fibroblast growth factor-2 arrests Mycobacterium avium sp. paratuberculosis growth and immunomodulates host response in macrophages." in: Tuberculosis (Edinburgh, Scotland), Vol. 95, Issue 4, pp. 505-14, (2015) (PubMed).

Hu, Zhang, Ren, Deng, Cai, Lei, Ping: "Effect of vacuum-assisted closure combined with open bone grafting to promote rabbit bone graft vascularization." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 1200-6, (2015) (PubMed).

Yu, Zhang, Jiang, Wang, Zhang: "Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction." in: Molecular medicine reports, Vol. 12, Issue 5, pp. 6718-26, (2015) (PubMed).

Ai, Liu, Sun: "Advanced glycation end-products stimulate basic fibroblast growth factor expression in cultured Müller cells." in: Molecular medicine reports, Vol. 7, Issue 1, pp. 16-20, (2014) (PubMed).

Zhou, Ma, Si, Li, Xu, Tu, Wang: "MicroRNA-503 targets FGF2 and VEGFA and inhibits tumor angiogenesis and growth." in: Cancer letters, Vol. 333, Issue 2, pp. 159-69, (2013) (PubMed).

Target Details for FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: FGF2

Background:

Synonyms: Fibroblast growth factor 2,FGF-2,Basic fibroblast growth factor,bFGF,Heparin-binding growth factor 2,HBGF-2,FGF2,FGFB,

Tissue Specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. .

Background: FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96 % and 97 % amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

Sequence Similarities: Belongs to the heparin-binding growth factors family.

Molecular Weight: 17-21 kDa

UniProt: P09038

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins